Journal: Toxins

Article Title: Efficacy of Fumonisin Esterase in Piglets as Animal Model for Fumonisin Detoxification in Humans: Pilot Study Comparing Intraoral to Intragastric Administration

doi: 10.3390/toxins14020136

Figure Lengend Snippet: Structures of ( a ) fumonisin B1 (FB1), ( b ) partially hydrolysed FB1a (pHFB1a), ( c ) pHFB1b, ( d ) hydrolysed FB1 (HFB1 or aminopentol), ( e ) sphinganine (Sa) and ( f ) sphingosine (So).

Article Snippet: Maltodextrin (placebo) and fumonisin esterase (10 U/g, FUM zyme ® ) were obtained from BIOMIN Holding GmbH (Tulln and Getzersdorf, Austria).

Techniques:

Journal: Toxins

Article Title: Efficacy of Fumonisin Esterase in Piglets as Animal Model for Fumonisin Detoxification in Humans: Pilot Study Comparing Intraoral to Intragastric Administration

doi: 10.3390/toxins14020136

Figure Lengend Snippet: Biomarkers for fumonisin B1 (FB1) exposure and effect measured in serum and faeces after administration of FB1 and fumonisin esterase to pigs.

Article Snippet: Maltodextrin (placebo) and fumonisin esterase (10 U/g, FUM zyme ® ) were obtained from BIOMIN Holding GmbH (Tulln and Getzersdorf, Austria).

Techniques:

Journal: Toxins

Article Title: Efficacy of Fumonisin Esterase in Piglets as Animal Model for Fumonisin Detoxification in Humans: Pilot Study Comparing Intraoral to Intragastric Administration

doi: 10.3390/toxins14020136

Figure Lengend Snippet: Mean sphinganine/sphingosine ratio (Sa/So) determined in pig serum during 24 h post-administration (p.a.) of a single intraoral administration of fumonisin B1 (2 mg/kg BW), either with a placebo (control group, n = 7), or with fumonisin esterase intraoral ( n = 8) or intragastric ( n = 7) administration. Error bars are the 95% confidence intervals. The asterisks represent a significant statistical difference ( p < 0.05) compared to the placebo group.

Article Snippet: Maltodextrin (placebo) and fumonisin esterase (10 U/g, FUM zyme ® ) were obtained from BIOMIN Holding GmbH (Tulln and Getzersdorf, Austria).

Techniques: Control

Journal: Toxins

Article Title: Efficacy of Fumonisin Esterase in Piglets as Animal Model for Fumonisin Detoxification in Humans: Pilot Study Comparing Intraoral to Intragastric Administration

doi: 10.3390/toxins14020136

Figure Lengend Snippet: Mean concentration-time curve (+standard error of the mean, SEM) of ( a ) fumonisin B1 (FB1), ( b ) hydrolysed fumonisin B1 (HFB1), ( c ) partially hydrolysed fumonisin B1a (pHFB1a) and ( d ) pHFB1b, determined in pig serum after a single intraoral administration of FB1 (2 mg/kg BW), either with a placebo (control group, n = 7, blue curve), with fumonisin esterase intraoral ( n = 8, orange curve) or intragastric ( n = 7, gray curve) administration. The scale of the y-axis is different for individual plots.

Article Snippet: Maltodextrin (placebo) and fumonisin esterase (10 U/g, FUM zyme ® ) were obtained from BIOMIN Holding GmbH (Tulln and Getzersdorf, Austria).

Techniques: Concentration Assay, Control

Journal: Toxins

Article Title: Efficacy of Fumonisin Esterase in Piglets as Animal Model for Fumonisin Detoxification in Humans: Pilot Study Comparing Intraoral to Intragastric Administration

doi: 10.3390/toxins14020136

Figure Lengend Snippet: Mean ± standard error of the mean (SEM) of toxicokinetic parameters for fumonisin B1 (FB1) and its hydrolysed variants determined in serum after single oral administration of FB1 (2 mg/kg BW) to pigs, combined with either a placebo (control group, n = 7), or fumonisin esterase administered intraorally ( n = 8) or intragastrically ( n = 7).

Article Snippet: Maltodextrin (placebo) and fumonisin esterase (10 U/g, FUM zyme ® ) were obtained from BIOMIN Holding GmbH (Tulln and Getzersdorf, Austria).

Techniques: Control, Concentration Assay

Journal: Toxins

Article Title: Efficacy of Fumonisin Esterase in Piglets as Animal Model for Fumonisin Detoxification in Humans: Pilot Study Comparing Intraoral to Intragastric Administration

doi: 10.3390/toxins14020136

Figure Lengend Snippet: Mean concentration-time bar chart (+ standard error of the mean, SEM) of ( a ) fumonisin B1 (FB1), ( b ) hydrolysed fumonisin B1 (HFB1), ( c ) partially hydrolysed fumonisin B1 a (pHFB1a) and ( d ) pHFB1b, determined in pig faeces post-administration (p.a.) of a single intraoral administration of FB1 (2 mg/kg BW) either with a placebo (control group, n = 7, blue bar), or with fumonisin esterase intraoral ( n = 8, orange bar) or intragastric ( n = 7, gray bar) administration. The scale of the y-axis is different for individual plots.

Article Snippet: Maltodextrin (placebo) and fumonisin esterase (10 U/g, FUM zyme ® ) were obtained from BIOMIN Holding GmbH (Tulln and Getzersdorf, Austria).

Techniques: Concentration Assay, Control

Journal: Toxins

Article Title: Efficacy of Fumonisin Esterase in Piglets as Animal Model for Fumonisin Detoxification in Humans: Pilot Study Comparing Intraoral to Intragastric Administration

doi: 10.3390/toxins14020136

Figure Lengend Snippet: Mean ± standard error of the mean (SEM) of toxicokinetic parameters for fumonisin B1 (FB1) and its hydrolysed variants determined in faeces after single oral administration of FB1 (2 mg/kg BW) to pigs, combined with either a placebo (control group, n = 7), or fumonisin esterase administered intraorally ( n = 8) or intragastrically ( n = 7).

Article Snippet: Maltodextrin (placebo) and fumonisin esterase (10 U/g, FUM zyme ® ) were obtained from BIOMIN Holding GmbH (Tulln and Getzersdorf, Austria).

Techniques: Control, Concentration Assay

Journal: Toxins

Article Title: Efficacy of Fumonisin Esterase in Piglets as Animal Model for Fumonisin Detoxification in Humans: Pilot Study Comparing Intraoral to Intragastric Administration

doi: 10.3390/toxins14020136

Figure Lengend Snippet: Overview of the administered products, doses per kg body weight (BW) and the administration route per treatment group.

Article Snippet: Maltodextrin (placebo) and fumonisin esterase (10 U/g, FUM zyme ® ) were obtained from BIOMIN Holding GmbH (Tulln and Getzersdorf, Austria).

Techniques: Maltodextrin

Journal: Toxins

Article Title: Efficacy of Fumonisin Esterase in Piglets as Animal Model for Fumonisin Detoxification in Humans: Pilot Study Comparing Intraoral to Intragastric Administration

doi: 10.3390/toxins14020136

Figure Lengend Snippet: Sampling points in hours after fumonisin B1 (FB1) and treatment administration. Blood and faeces were collected at the different times indicated. Treatment consisted of fumonisin esterase administration intraorally or intragastrically, or maltodextrin (a placebo).

Article Snippet: Maltodextrin (placebo) and fumonisin esterase (10 U/g, FUM zyme ® ) were obtained from BIOMIN Holding GmbH (Tulln and Getzersdorf, Austria).

Techniques: Sampling, Maltodextrin

Journal: Frontiers in Microbiology

Article Title: Characterization of Influenza A Virus Infection in Mouse Pulmonary Stem/Progenitor Cells

doi: 10.3389/fmicb.2019.02942

Figure Lengend Snippet: Profiles of releasing cytokines and chemokines from PR8-infected mouse pulmonary stem/progenitor cells (mPSCs), mPSCs Oct4+ E3L clone, AT-I, and MLE15 cells. (A) Fold changes of cytokines and chemokines released from PR8-infected cells. Cells were infected with PR8 at a multiplicity of infection (MOI) of 10. The cultured supernatants were harvested from mock-infected or PR8-infected cells at 12 hpi. Profiles of releasing cytokines and chemokines in the cultured supernatants were determined by the cytokine array. The fold changes of each cytokine or chemokine between virus- and mock-infected cells were shown. (B) Release of interferon (IFN)-β from the PR8-infected mPSCs, mPSCs Oct4+ E3L clone, AT-I, and MLE15 cells. The cells were infected with PR8 at an MOI of 10. Concentration of IFN-β in the culture supernatants collected at 0, 12, 24, 40, or 48 hpi was determined by ELISA. (C) Induction of IFN-β mRNA expression in the mPSCs, mPSCs Oct4+ E3L clone, AT-I, MLE15, and Madin-Darby canine kidney (MDCK) cells after PR8 infection. Total RNA was extracted from PR8-infected cells at 0, 3, 6, 12, and 24 hpi. mRNA of IFN-β and GAPDH was quantified by RT-PCR. The expression of IFN-β mRNA was normalized with GAPDH mRNA. The ratios shown were normalized to 0 hpi. At least three independent experiments were performed, and the ratios were presented as mean ± SD. N.D., none detected.

Article Snippet: For determination of IFN-β expression, the VeriKine-HS TM mouse IFN beta serum ELISA Kit was used (42410, pbl Assay Science; United States).

Techniques: Infection, Cell Culture, Virus, Concentration Assay, Enzyme-linked Immunosorbent Assay, Expressing, Reverse Transcription Polymerase Chain Reaction